Automated Tissue Cell Processing Lab
KISO Processor
Automated Cell Lab
KEAI Bioresearch Inc. is a biotechnology company pushing the frontiers of medical research and clinical sciences. Our vision started 5 years ago with the belief that innovative thinking, bold and courageous leadership, personal dedication and the utilization of cutting edge technology would result in the creation of a platform technology that could change the future of medical research and in vitro diagnostics.
Introducing KISO system
The KISO system allows physicians, diagnostic laboratories and researchers to take human tissue and automatically isolate a consistent, high quality, potent unit of rich regenerative cells, cell isolates or target microorganisms for in vitro diagnostics. The entire process is completely standardized and automated in a closed system.
Compact & Standardized
Our easy to use proprietary patented technology is designed for applications where safe, reproducible, quality controlled products are desired. This results in superior recovery of minimally manipulated cells of pristine quality and purity within an hour of tissue harvesting.
What We Do
Demand Only the Best Cells and Best Prepared Tissue Samples for Invitro Diagnostics
Highest Quality Cells, Clump Free! (left)
Our system provides highly potent & viable stem cells for the best possible outcome. Say good bye to cell clumps and clogged needles!
Our multi-step processing (enzyme or enzyme free) provides the best cells possible. This means cleaner research projects and a smoother mixture of regenerative cells for a wide range of applications.
Competitor's Output (right)
Clumps of dead cells are not desired and will jam needles on injections or cause serious process complications
Real Cell Counts vs Cell Counts
Not all count data provided is comparable
A cell count should provide you with viable nucleated cells and not a total count that includes non-viable cells. Additionally, competitors samples are known to be highly contaminated with a high level of white blood cells - artificially increasing the cell counts. Additionally, the majority of modern culture techniques still take a colony-forming unit-fibroblasts (CFU-F) approach for determining the count of Mesenchymal stem cells, or MSCs - that can differentiate into a variety of cell types. This MSC count data is often unavailable from competitors and providers
of stem cell procedures as it has been shown in academic literature to be very low vs our high MSCs counts.
Viable Nucleated Cells
Ask for the counts. I'll show you mine - you show me yours! Don't settle for less.
801,000 ± 167,000 viable nucleated cells from each gram of human lipoaspirate tissue (mean ± SD, n=7, median: 786,000 viable nucleated cells/g), with a range of 570,000 – 1,060,000 viable nucleated cells/g. The viability of cells recovered: 84.8% ± 3.8%. The high viability is an indicator and the gentle handling and isolation of the cells.
Higher viability means gentler handling of the cells, greater potency -> significant medical outcomes. New trials with our platform will lead to new outcomes never seen before.
7.2% ± 2.4% or 57,700 MSCs/g of the viable nucleated cells in the output our our system are cells MSCs as characterized by colony-forming unit-fibroblasts (“CFU-F”), compared to the reported 0.2% – 1.7% achieved by competitor systems. With the significantly higher viable cell recovery and stem cell colony counts, our system can produce 14x to 640x more MSCs, from each gram of human lipoaspirate vs the leading competitors.
Extreme Standardization
Regardless of where research or cell therapy is conducted, medicine requires standardization and performance reproducibility.
Our resulting measure of machine-to-machine variability was 2.4%, suggesting that the systems can perform stem cell extraction with exceptional consistency and reproducibility - eliminating any doubt in whether you are getting stem cells or not in your procedure.
Ease of Use and Speed Matters
Fast and furious "gentle" processing in 50 minutes
Extremely easy to operate with basic orientation and training. Once the isolation process starts, walk away and wait for you cells to be freshly and gently isolated.
Unrealized until now.
Indispensable from now on.
A better, faster stem cell processor.
22x
KISO produces over 22X more viable cells per gram of adipose tissue compared to Lipokit.+
KISO extracts as many cells from 60ml of adipose tissue as Lipokit does from 1 liter – automatically, safely, and at a lower total cost.*
+Saving costs include labour, capital costs, sterilization, disposables, admin efficiencies and reduced quantity of reagents (research or GMP grade).
*KISO competitor data from J. Aronowitz, Plast Reconstr Surg. 132(6):932e-9e, 2013
Recommended Options
Authorized Distributor Quality Invitro Diagnostic Accessories for your Lab
E8 Centrifuge
Swing-Out or Angled
The E8 Fixed is designed for blood separations at a fixed speed of 3500 rpm only (1534 g-force). The E8 Variable and E8 Digital will separate blood at 3500 rpm and can be slowed to separate other fluids at lower g-force, such as urine specimens as outlined by C.L.I.A. regulations.
The E8 has either a swing-out 6-place rotor spins up to six 3.0ml-4.5ml test tubes horizontally or an angled 8-place rotor spins up to eight 3.0ml-15ml test tubes.
Universal Centrifuge
Authorized Distributor
With the choice of either an 8 place fixed-angle rotor or a 6 place swing-out rotor, the Universal Centrifuge is the perfect solution for both laboratories and doctors’ offices. This centrifuge features a ZERO-RPM locking lid for safety and digital programming of time and speed ranging from 800-3400rpm. Achieve up to 1450g with the fixed angle rotor and 1809g with the swing-out rotor resulting in clean, easy to read lines of separation. The Universal centrifuge has a 110-220v auto-switching power adapter.
Maximum Tube Sizes:
- 8-place fixed-angle rotor: 122mm tall and 17.5mm wide if spinning 8 tubes (full load), and 131mm tall and 17.5mm wide if spinning 4 tubes (every other position).
- 6-place swing-out rotor: 131mm tall and 17.5mm wide.
- 40-place microtube rotor: 11mm wide (at the collar) and 50mm tall.
- 4-place, 50ml rotor: 30mm wide and 120mm tall.
Applications: Blood, Urine, Semen, Fecal
LW Scientific Incubators
Authorized Distributor
The digitally controlled Incubators are perfect for various applications such as cultures, egg incubation, test kits, fluid bag warming, and other microbiological uses in pediatric, physician, and hospital labs. This line of incubators are designed, engineered, and assembled in the USA by LW Scientific Atlanta. They provide better temperature stability and accuracy with the digital PID microprocessor controls, and the convection heat provides excellent heat distribution and uniformity. The LED temperature display shows constant temperature in Celsius or Fahrenheit, and the aluminum and stainless steel components are corrosion resistant and easy to clean.
The LW Scientific Incubator is perfect for labs requiring better temperature accuracy and a small footprint.
Culture Media & Reagents
Authorized distributor for Cell Systems Corp. - recommended for all your in vitro diagnostic and microbiological cultures.
Standard Tools For Cell Biology
Cell Systems Corporation has, since 1991, focused on developing and manufacturing standard research tools for the science of cell biology.Cell Systems manufactures as a gold standard, normal primary CSC Certified cells. Using as a reference these cell lines, ACBRI formulated and qualified complete media and reagents appropriate for use as delivered with >90% of cell types in use today.
The importance of the CSC medium formulation is best understood by reviewing formulation dates for the mediums in use in laboratories right now : M1991 (1950-52), BME2 (1955-76), DMEM3 (1959), F-124(1965), and MCDB5 (1976).
These formulations must be variously supplemented, modified and tweaked to support the housekeeping metabolism of each different cell type. Additional products must be added for growth, and decisions made regarding serum, concentration, testing and cost.
Chances are that the only components of cell biology research with a more venerable heritage are Earle's6 (1943) and Hank's7 (1949) salt mixture
Its now time that recent knowledge be incorporated in the formulation of media and reagents qualified for use with standard cells. This is the logic that governs and focuses CSC Certified™ Cells, Medium Kits and Reagents
Medium Formulation References:
1. Morgan, J.F., Morton, H.J., and Parker, R.C. (1950) Proc. Soc. Exp. Biol. Med. 73: 1-8
2. Morgan, J.F., Campbell, E., and Morton, H.J. (1955) J. Nat. Cancer Inst. 16(2): 557-567
3. Basal Medium Eagle, Eagle, H. et. al, J. Biol. Chem. (1956) 214: 839
4. Eagle H. Tissue Culture Association Manual, (1976) 3: 517-520
5. Dulbecco's Modified Eagle's Medium (DMEM), Dulbecco, R., and Freeman, G., Virology (1959) 8: 396-397
6. Nutrient Mixture F-12, Ham, R.G., Proc. Nat. Acad. Sci. (1965) 53: 288-293
7. MCDB Medium McKeehan, W.L., and Ham, R.G., In Vitro (1976) 13(9): 537-547.
